Safranin O-stained antigen microagglutination test for detection of brucella antibodies.
نویسندگان
چکیده
A microagglutination test was compared with a standard tube agglutination test for the detection of brucella antibodies. Advantages of the microagglutination test were that it required less time to perform, had a shorter incubation period, and used less antigen.
منابع مشابه
Serological survey of the wild boar (Sus scrofa) for tularaemia and brucellosis in South Moravia, Czech Republic
Sera of 204 wild boars (Sus scrofa), shot by hunters in the South-Moravian district of Břeclav during 19932001, were tested by microagglutination reaction using safranin-stained antigens of Francisella tularensis and Brucella abortus: 10.8% and 8.7% seroreactors, respectively, were detected. The highest (17%) prevalence of tularaemia antibodies was found in wild boars during 1993–1994 at the be...
متن کاملMicroagglutination procedures for febrile agglutination tests.
Febrile agglutination tests were done by using as antigens Brucella abortus, Salmonella group D, Proteus OX19, and Pasteurella tularensis. Comparison of results from 23 sera showed that the microtechnique, rapid slide, and test tube methods gave similar titers, although those from the microtechnique were generally higher. The sensitivity of the microtechnique depended upon the concentration of ...
متن کاملCross-reaction to Legionella pneumophila antigen in sera with elevated titers to Pseudomonas pseudomallei.
A significantly greater incidence (P less than 0.005) of Legionella pneumophila microagglutination titers greater than or equal to 32 was found in sera with elevated titers to Pseudomonas pseudomallei as compared with sera with negative titers for P. pseudomallei antibodies. The greater incidence of L. pneumophila titers in these sera suggests that L. pneumophila and P. pseudomallei share an an...
متن کاملSeroepidemiology and molecular detection of Brucella infection in Iranian horses: Aprovincial study
BACKGROUND: Brucellosis is a febrile zoonotic infectionand has worldwide distribution among humans as well asanimals. Although the seroprevalence of brucellosis in variousanimals has been described in Iran, there is only one report onequine brucellosis in the region. OBJECTIVES: This study wascarried out to determine the seroprevalence of brucellosis inracing clubs and private horse owners in t...
متن کاملEffect of centrifugation and microagglutination techniques on Brucella agglutinin titers.
The microagglutination technique without centrifugation was more effective than centrifugation of the standard tube test for increasing Brucella agglutinin titers of specimens with a titer greater than or equal to 160 but was less effective than centrifugation of the standard tube test for specimens with a titer less than 160.
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of clinical microbiology
دوره 13 2 شماره
صفحات -
تاریخ انتشار 1981